Immotile sperm effect on cells

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Sperm and Semen Testing and Evaluation

Antiretroviral therapy reduces sperm motility, Dutch study finds | aidsmap

In many fish species, sperm cryopreservation has deleterious effects and leads to a significant decrease in spermatozoa viability. However, the effect of cryopreservation on sperm cells that survive this process and are still viable is not fully understood. The objective of this study was to compare the viability and proteomes of fresh and cryopreserved sterlet Acipenser ruthenus sperm samples before and after live-dead cell separation using Percoll density gradient centrifugation. Both fresh and cryopreserved sperm samples were divided into two groups with or without application of Percoll separation. At each step of the experiment, sperm quality was evaluated by video microscopy combined with integrated computer-assisted sperm analysis software and flow cytometry for live-dead sperm viability analysis. Straight line velocity and linearity of track were significantly lower in cryopreserved samples than in those separated by Percoll before and after cryopreservation. However, only one protein spot was significantly altered when samples before and after cryopreservation followed by Percoll separation were compared.
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Sperm motility

Frente, Porto, Portugal. Alameda Prof. The genetic bases and molecular mechanisms involved in the assembly and function of the flagellum components as well as in the regulation of the flagellar movement are not fully understood, especially in humans. There are several causes for sperm immotility, of which some can be avoided and corrected, whereas other are related to genetic defects and deserve full investigation to give a diagnosis to patients.
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Tutor A. Spermatozoa were first described by Leeuwenhoek in the 17th century but it was not until that the sperm count was found to be associated with fertility potential Since that time a variety of sperm tests and semen parameters have been developed with the hope of clarifying whether or not a man could impregnate his partner. MacLeod , MacLeod and Gold , Eliasson and Hellinga , have led the scientific basis of conventional analysis of spermatozoa and the techniques recommended by them are still considered the reference for more advanced methods 6. Semen analysis comprises a set of descriptive measurements of spermatozoa and seminal fluid parameters that help to estimate semen quality 4.
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