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GenoMass software: a tool based on electrospray ionization tandem mass spectrometry for characterization and sequencing of oligonucleotide adducts

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The analysis of DNA adducts is of importance in understanding DNA damage, and in the last few years mass spectrometry MS has emerged as the most comprehensive and versatile tool for routine characterization of modified oligonucleotides. The structural analysis of modified oligonucleotides, although routinely analyzed using mass spectrometry, is followed by a large amount of data, and a significant challenge is to locate the exact position of the adduct by computational spectral interpretation, which still is a bottleneck. These efforts have attained a high degree of success in the field of proteomics with the development of several data mining software on the basis of database-driven approaches or the de novo sequencing approach. However, in contrast, progress in the field of genomics has been generally slow, and limited success has been achieved in the development of similar software packages. These fragments are also routinely used to locate the exact position of modification in oligonucleotides. The first one was reported in by Rozenski and McCloskey when they presented an automated sequencing algorithm, simple oligonucleotide sequencer SOS , a user-interactive local search algorithm for ab initio determination of unknown oligonucleotides for 20 bases or less.
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